Scientist I – T-Cell Engineering & Functional Assays

🧬 WE’RE HIRING: Scientist I – T-Cell Engineering & Functional Assays
📍 Location: San Diego, CA 92111 (Onsite)
⏳ Duration: 7-Month Contract | High Possibility of Extension
Are you passionate about immunology, T-cell engineering, and cutting-edge therapeutic discovery? Join our Discovery Biology team and contribute to a high-priority binder screening campaign focused on advancing novel candidates for next-generation T-cell-based therapies!
We are looking for a motivated, detail-oriented Scientist I with strong hands-on wet lab expertise and a passion for experimental science.
🔬 Key Responsibilities
Perform non-viral engineering of primary human T cells using electroporation or nucleofection platforms.
Isolate, culture, maintain, and expand primary human T cells and Jurkat cell lines.
Execute high-throughput binder screening and functional validation assays.
Perform multi-color flow cytometry (FACS) for surface expression and phenotypic analysis using FlowJo.
Conduct T-cell activation, cytotoxicity, and proliferation assays using IncuCyte, LDH release, and CFSE-based methods.
Perform cytokine profiling using ELISA, MSD, or Luminex platforms.
Maintain accurate experimental records, raw data, and protocol deviations in an Electronic Lab Notebook (ELN).
Present clear, data-driven updates during weekly campaign meetings.
🎓 Required Qualifications
Bachelor’s or Master’s degree in Immunology, Molecular Biology, Bioengineering, or a related life science field.
2+ years of hands-on wet lab experience, preferably in the biotechnology or pharmaceutical industry.
Strong expertise in aseptic techniques and primary T-cell isolation from PBMCs.
Proven experience in flow cytometry acquisition, analysis, and data interpretation.
Experience with mammalian cell culture and functional immunology assays.
⭐ Preferred Skills
Hands-on experience with non-viral transfection of primary T cells.
Familiarity with mRNA delivery, transposon-based systems, electroporation, or nucleofection.
Experie